Neural stem cell differentiation into mature neurons: Mechanisms of regulation and biotechnological applications. Neural stem cells: Progression of basic research and perspective for clinical application. Current progress in the derivation and therapeutic application of neural stem cells. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. * p < 0.05 and ** p < 0.01: (NSCiM1 NSC induction medium 1) vs differentiation media.Īdult stem cells Myelination Neural differentiation Neural stem cells Transdifferentiation. Two-way ANOVA with Bonferroni post-test with n = 3. NES: nestin GFAP: Glial fibrillary acidic protein. Inferior part: Neural phenotype fates in diverse differentiation media. The nuclear staining in blue corresponds to DAPI. Superior Part: Schematic organization of obtaining and generating hNSC from hADSC and differentiation processes and phenotypic expression of neuron, astrocyte and oligodendrocyte markers (MAP2, GFAP and O4, respectively) and stem cell marker (NES) of differentiating hNSC 14 days after induction. We show here that hADSC can be transdifferentiated into NSC and distinct neural phenotypes with the occurrence of neuron myelination in vitro, providing novel strategies for CNS regeneration therapy. Co-cultured NLC and OLC showed excitability in 81.3% of cells and 23.5% of neuron/oligodendrocyte marker expression overlap indicating occurrence of in vitro myelination. Accordingly, immunostaining for nestin + in NSC, GFAP + in astrocytes and GalC/O4 + in oligodendrocytes was detected. Following differentiation induction into NLC, OLC or ALC, specific neural phenotypes were obtained expressing MAP2, GalC/O4 or GFAP with compatible morphologies, respectively. NSC showed augmented gene expression of NES, TUJ1, GFAP and PDGFRA/Cnp. Our methodologies were capable of producing NLC, ALC and OLC from adult murine and human transdifferentiated NSC. For both cell types, their capabilities of differentiating into neuron-, astrocyte- and oligodendrocytes-like cells (NLC, ALC and OLC, respectively) were analyzed. NSC from adult mouse brains and human adipose-derived stem cells (hADSC) were isolated and characterized regarding their neural differentiation potential based on neural marker expression profiles. Here, we describe novel neural differentiation methods. In this regard, adult neural stem cells (NSC) are studied for their mechanisms of proliferation, differentiation and functionality in neural repair. cell therapy is an interesting approach for neural repair, once it can improve and increase processes, like angiogenesis, neurogenesis, and synaptic plasticity. 7 Laboratório de Sinalização Celular e Nanobiotecnologia, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. 6 Laboratório de Neurociências, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil. 5 Institute of Clinical Immunology, Universitätsklinikum Leipzig, Leipzig, Germany.1 Laboratório de Sinalização Celular e Nanobiotecnologia, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. 4 Institute of Clinical Immunology, Universitätsklinikum Leipzig, Leipzig, Germany.3 Departamento de Patologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.2 Laboratório de Neuroquímica e Neurofarmacologia, Departamento de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.1 Laboratório de Sinalização Celular e Nanobiotecnologia, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
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